Tuesday, June 22, 2010

your mom doesn't work here....

clean up after yourself.

Today I was imaging some stuff experiments I did last week. I work with mitotic chromosomes so the way I fix my samples there should be no microtubules in my preps. Some times I will get a spindle or two that didn't break up fully but nothing more than that. Today while imaging I noticed a lot of free microtubules and perfectly round glowing blobs. I figured maybe I didn't mix all my samples up correctly and got the MTs in there but the blobs were confusing. Come to find out they are beads from an experiment that someone else did before me. We share glass test tubes that are used to spin our experiments onto cover slips (spin-downs). Someone (I know who... fucker) did not clean their tubes at all. Just turned them over in the rack to dry. This means when I'm an idiot and assume that the tubes that are flipped over in the rack are clean and dry I get all your left over shit on my cover slips. Lesson learned on my part... clean the tubes before doing spin downs, but god damn it.

Don't be an asshole. Clean up your shit.

Friday, June 18, 2010

-80 thaw

Get the fuck out of my way when I have a 20 pound, 3 feet long piece of incredibly cold metal in my hands. I will hurt you with it (perhaps unintentionally... perhaps not).

That is all.